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13 March 2024 Development of turn-key lasers for three-photon microscopy
Jonas Berzinš, Mantvydas Mikulis, Lukas Kontenis
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Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470A (2024) https://doi.org/10.1117/12.3003079
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Multiphoton imaging typically requires a complex multi-device laser system, a large optical table, and skilled staff. This reality encumbers neuroscience and other biomedical applications. Here, we will present our direction to provide a turn-key solution for three-photon (3P) imaging. The application-optimized laser provides the anticipated reliability and simplicity but also maintains tunability in wavelength and dispersion control, which seems quite important in the current state of the application. We will explicitly dig into details of long-term power and pulse-to-pulse energy stability, and how that directly impacts your imaging results.
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Jonas Berzinš, Mantvydas Mikulis, and Lukas Kontenis "Development of turn-key lasers for three-photon microscopy", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470A (13 March 2024); https://doi.org/10.1117/12.3003079
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KEYWORDS
Laser development
Multiphoton microscopy
Biomedical applications
Biomedical optics
Imaging systems
Neuroscience
Optical parametric amplifiers
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