Multibeam two-photon microscopy for multispectral ultrahigh-throughput imaging

Vincent D. Ching-Roa; Chi Z. Huang; Michael Giacomelli

doi:10.1117/12.3001696

13 March 2024 Multibeam two-photon microscopy for multispectral ultrahigh-throughput imaging

Vincent D. Ching-Roa, Chi Z. Huang, Michael Giacomelli

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470K (2024) https://doi.org/10.1117/12.3001696
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

A multiplane two-photon microscope with non-descanned detection provides imaging speeds up to 200 MP/s with four spectral channels (800 MSpectra/s) with area imaging speeds of up to 30 mm2/s. This enables high volumetric throughput deep-tissue imaging at speeds approaching light-sheet imaging without sacrificing resilience to scattering and high axial resolution. Multiplane excitation is achieved by multifocal scanner-synchronous strip-scanning with tilted translation, while spherical lens arrays with custom SiPM array boards provide non-descanned detection that is both inexpensive and easy to spectrally multiplex.

Conference Presentation

Citation Download Citation

Vincent D. Ching-Roa, Chi Z. Huang, and Michael Giacomelli "Multibeam two-photon microscopy for multispectral ultrahigh-throughput imaging", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470K (13 March 2024); https://doi.org/10.1117/12.3001696

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