Ever faster FLIM: why speed and sensitivity are no contradiction (anymore)

Julius Heitz; Lukas Braun; Taravat Saeb-Gilani; Hauke Studier; Wolfgang Becker

doi:10.1117/12.3005144

13 March 2024 Ever faster FLIM: why speed and sensitivity are no contradiction (anymore)

Julius Heitz, Lukas Braun, Taravat Saeb-Gilani, Hauke Studier, Wolfgang Becker

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470U (2024) https://doi.org/10.1117/12.3005144
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Fluorescence lifetime imaging (FLIM) is a popular and versatile tool for in vivo research with minimal disruption of the biological system. Recently, the desire has grown to investigate dynamic processes with FLIM, creating a need for video rate FLIM contrast measurements. Because the potential applications are manifold, the different information depth of video FLIM and ‘detailed, slow’ FLIM measurements must be considered. This talk highlights the complimentary nature of video rate and standard FLIM measurements with practical examples, measured with the same machine. Strengths and limitations of each measurement mode are highlighted, and best-practice analysis strategies are determined for each.

Conference Presentation

Citation Download Citation

Julius Heitz, Lukas Braun, Taravat Saeb-Gilani, Hauke Studier, and Wolfgang Becker "Ever faster FLIM: why speed and sensitivity are no contradiction (anymore)", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470U (13 March 2024); https://doi.org/10.1117/12.3005144

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