Stimulated Raman imaging device for histology of fresh tissue samples from 700 to 3300 wavenumbers

Maximilian Brinkmann; Maryam Rezaei; Ramon Droop; Christoph Engwer; Felix Neumann; Niklas Lüpken; Sven Dobner; Tim Hellwig

doi:10.1117/12.3001389

13 March 2024 Stimulated Raman imaging device for histology of fresh tissue samples from 700 to 3300 wavenumbers

Maximilian Brinkmann, Maryam Rezaei, Ramon Droop, Christoph Engwer, Felix Neumann, Niklas Lüpken, Sven Dobner, Tim Hellwig

Author Affiliations +

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128551D (2024) https://doi.org/10.1117/12.3001389
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We present a fully integrated, clinical-compatible SRS imaging device giving access to the complete Raman spectrum during tumor surgeries. Leveraging the advantages of a compact and robust fiber laser, we have integrated the entire microscopy system into a clinical cart, facilitating deployment in diverse clinical environments. The laser provides rapid tunability within milliseconds across a broad spectral range of 700 to 3300 cm^-1, covering biomedically relevant resonances in the fingerprint region. For detailed examination of larger tissue samples, we have designed a high-speed, low-resolution imaging mode to quickly identify cancerous hot-spots, followed by a high-resolution imaging mode.

Conference Presentation

Citation Download Citation

Maximilian Brinkmann, Maryam Rezaei, Ramon Droop, Christoph Engwer, Felix Neumann, Niklas Lüpken, Sven Dobner, and Tim Hellwig "Stimulated Raman imaging device for histology of fresh tissue samples from 700 to 3300 wavenumbers", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128551D (13 March 2024); https://doi.org/10.1117/12.3001389

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