Yanis Taege,1 Madhu Veettikazhy,2 Tim Samuel Winter,1 Stefan Mark Jensen,2 Sophia Laura Stark,3 Anja Lykke Borre,2 Lars Rene Lindvold,2 Bernhard Messerschmidt,3 Dominik Martihttps://orcid.org/0000-0002-1745-3116,2 Peter Eskil Andersen,2 Çaglar Ataman1
1Univ. of Freiburg (Germany) 2Technical Univ. of Denmark (Denmark) 3Grintech GmbH (Germany)
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We introduce a compact attachment for microscope objectives that allows for the conversion of conventional fluorescence microscopes into Airy light-sheet microscopes. The attachment includes a one-dimensional Airy beam generator, which comprises a gradient-index collimator and a 3D nano-printed cubic phase-plate, realized through two-photon polymerization 3D nano-printing and a two-step writing process that guarantees an optical-quality surface for the phase plate. The micro-optical unit is affixed to a mechanical holder equipped with micro-stages, thereby facilitating the unit's integration into commercial microscopes. The implementation and imaging performance of this system and its fundamental imaging characteristics are discussed, with findings based on diverse samples.
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Yanis Taege, Madhu Veettikazhy, Tim Samuel Winter, Stefan Mark Jensen, Sophia Laura Stark, Anja Lykke Borre, Lars Rene Lindvold, Bernhard Messerschmidt, Dominik Marti, Peter Eskil Andersen, Çaglar Ataman, "Miniaturized fiber-based Airy light-sheet generator with a 3D nano-printed freeform optical phase plate," Proc. SPIE PC12995, 3D Printed Optics and Additive Photonic Manufacturing IV, PC1299506 (18 June 2024); https://doi.org/10.1117/12.3021695