Interferometric Gated Off-Axis Reflectometry (iGOR): ultrasensitive label-free tracking of nanoparticles and suspended membranes in three dimensions

Wolfgang W. Langbein; Freya Turley; David Regan; Paola Borri

doi:10.1117/12.3021988

18 June 2024 Interferometric Gated Off-Axis Reflectometry (iGOR): ultrasensitive label-free tracking of nanoparticles and suspended membranes in three dimensions

Wolfgang W. Langbein, Freya Turley, David Regan, Paola Borri

Author Affiliations +

Proceedings Volume PC12996, Unconventional Optical Imaging IV; PC129960B (2024) https://doi.org/10.1117/12.3021988
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

To unravel dynamic processes underpinning key functions in cell biology, it is essential to develop imaging technologies able to track the movement of individual bio-nano-objects under physiologically relevant conditions, hence at high speed (ms) and in 3D. We demonstrate interferometric gated off-axis reflectometry (iGOR) which detects the back-scattered light of the structure of interest using an external off-axis reference, enabling label-free high-speed tracking of nanoparticles and suspended membranes in 3D volumes. Employing coherence time-gating by femtosecond pulses, the axial extension of the detected volume is controlled. We show tracking of single nanoparticles down to 10nm size freely diffusing in volume, which allows us to determine their geometrical and hydrodynamic radius as well as non-sphericity. We also show the spatiotemporal dynamics of suspended lipid bilayers, and the influence of lipid phase transitions on these dynamics with sub-nm thickness precision.

Conference Presentation

Citation Download Citation

Wolfgang W. Langbein, Freya Turley, David Regan, and Paola Borri "Interferometric Gated Off-Axis Reflectometry (iGOR): ultrasensitive label-free tracking of nanoparticles and suspended membranes in three dimensions", Proc. SPIE PC12996, Unconventional Optical Imaging IV, PC129960B (18 June 2024); https://doi.org/10.1117/12.3021988

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