CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 13 > Issue 2 > Article
Open Access
1 March 2008 Pulsed-laser-induced damage in rat corneas: time-resolved imaging of physical effects and acute biological response
Anoop V. Cherian, Kaustubh R. Rau
Author Affiliations +
Journal of Biomedical Optics, Vol. 13, Issue 2, 024009 (March 2008). https://doi.org/10.1117/1.2907214
Abstract
Laser-induced damage is studied in the rat corneal epithelium and stroma using a combination of time-resolved imaging and biological assays. Cavitation bubble interactions with cells are visualized at a higher spatial resolution than previously reported. The shock wave is observed to propagate through the epithelium without cell displacement or deformation. Cavitation bubble expansion is damped in tissue with a reduction in maximum size in the range of 54 to 59%, as compared to 2-D and 3-D cultures. Bubble expansion on nanosecond timescales results in rupture of the epithelial sheet and severe compression of cell layers beyond the bubble rim. In the stroma, the dense collagen lamellae strongly damped bubble expansion, thus resulting in reduced damage. The acute biological response of this tissue to laser pulses is characterized by confocal fluorescence microscopy. A viability assay of the epithelium reveals that only cells around the immediate site of laser focus are killed, while cells seen to undergo large deformations remain alive. Actin morphology in cells facing this mechanical stress is unchanged. Collagen microstructure in the stroma as revealed by second-harmonic imaging around the ablation site shows minimal disruption. These cellular responses are also compared to in vitro 2-D and 3-D cell cultures.
©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Citation Download Citation
Anoop V. Cherian and Kaustubh R. Rau "Pulsed-laser-induced damage in rat corneas: time-resolved imaging of physical effects and acute biological response," Journal of Biomedical Optics 13(2), 024009 (1 March 2008). https://doi.org/10.1117/1.2907214
Published: 1 March 2008
JOURNAL ARTICLE
 10 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 20 scholarly publications and 1 patent.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Cavitation
Collagen
Tissues
Confocal microscopy
Cornea
Laser ablation
Laser tissue interaction
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top