Resonance Raman measurements of carotenoids using light-emitting diodes

Scott D. Bergeson; Justin B. Peatross; N. Jay Eyring; John F. Fralick; D. N. Stevenson; Scott B. Ferguson

doi:10.1117/1.2952075

1 July 2008 Resonance Raman measurements of carotenoids using light-emitting diodes

Scott D. Bergeson, Justin B. Peatross, N. Jay Eyring, John F. Fralick, D. N. Stevenson, Scott B. Ferguson

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Journal of Biomedical Optics, Vol. 13, Issue 4, 044026 (July 2008). https://doi.org/10.1117/1.2952075

Abstract

We report on the development of a compact commercial instrument for measuring carotenoids in skin tissue. The instrument uses two light-emitting diodes (LEDs) for dual-wavelength excitation and four photomultiplier tubes for multichannel detection. Bandpass filters are used to select the excitation detection wavelengths. The f/1.3 optical system has high optical throughput and single photon sensitivity, both of which are crucial in LED-based Raman measurements. We employ a signal processing technique that compensates for detector drift and error. The sensitivity and reproducibility of the LED Raman instrument compares favorably to laser-based Raman spectrometers. This compact, portable instrument is used for noninvasive measurement of carotenoid molecules in human skin with a repeatability better than 10%.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Scott D. Bergeson, Justin B. Peatross, N. Jay Eyring, John F. Fralick, D. N. Stevenson, and Scott B. Ferguson "Resonance Raman measurements of carotenoids using light-emitting diodes," Journal of Biomedical Optics 13(4), 044026 (1 July 2008). https://doi.org/10.1117/1.2952075

Published: 1 July 2008

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