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1 September 2008 Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin
Dan Fu, Tong Ye, Thomas E. Matthews, James M. Grichnik M.D., Lian Hong, John D. Simon, Warren S. Warren Sr.
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Journal of Biomedical Optics, Vol. 13, Issue 5, 054036 (September 2008). https://doi.org/10.1117/1.2976424
Abstract
As some of the most ubiquitous and biologically important natural pigments, melanins play essential roles in the photoprotection of skin. Changes in melanin production could potentially be useful for clinical diagnosis of the progression stage of melanoma. Previously we demonstrated a new method for imaging melanin distribution in tissue with two-color transient absorption microscopy. Here we extend this study to longer wavelengths and show that we are able to image melanin in fixed thin skin slices with higher signal-to-noise ratios (SNRs) and demonstrate epimode imaging. We show that both photothermal effects and long-lived excited states can contribute to the long-lived signal. Eumelanin and pheomelanin exhibit markedly different long-lived excited state absorption. This difference should enable us to map out their respective distribution in tissue samples with subcellular resolution. This technique could provide valuable information in diagnosing the malignant transformation of melanocytes.
©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Dan Fu, Tong Ye, Thomas E. Matthews, James M. Grichnik M.D., Lian Hong, John D. Simon, and Warren S. Warren Sr. "Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin," Journal of Biomedical Optics 13(5), 054036 (1 September 2008). https://doi.org/10.1117/1.2976424
Published: 1 September 2008
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Cited by 51 scholarly publications and 1 patent.
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KEYWORDS
Skin
Absorption
Melanoma
Tissues
Signal to noise ratio
Phase shift keying
Thermal effects
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