Light scattering by neutrophils: model, simulation, and experiment

Darya Yu. Orlova; Maxim A. Yurkin; Alfons G. Hoekstra; Valerii Pavlovich Maltsev

doi:10.1117/1.2992140

1 September 2008 Light scattering by neutrophils: model, simulation, and experiment

Darya Yu. Orlova, Maxim A. Yurkin, Alfons G. Hoekstra, Valerii Pavlovich Maltsev

Author Affiliations +

Journal of Biomedical Optics, Vol. 13, Issue 5, 054057 (September 2008). https://doi.org/10.1117/1.2992140

Abstract

We studied the elastic light-scattering properties of human blood neutrophils, both experimentally and theoretically. The experimental study was performed with a scanning flow cytometer measuring the light-scattering patterns (LSPs) of individual cells over an angular range of 5–60 deg. We determined the absolute differential light-scattering cross sections of neutrophils. We also proposed an optical model for a neutrophil as a sphere filled by small spheres and prolate spheroids that correspond to granules and segmented nucleus, respectively. This model was used in simulations of LSPs using the discrete dipole approximation and different compositions of internal organelles. A comparison of experimentally measured and simulated LSPs gives a good qualitative agreement in LSP shape and quantitative agreement in overall magnitude of the differential light-scattering cross section.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Darya Yu. Orlova, Maxim A. Yurkin, Alfons G. Hoekstra, and Valerii Pavlovich Maltsev "Light scattering by neutrophils: model, simulation, and experiment," Journal of Biomedical Optics 13(5), 054057 (1 September 2008). https://doi.org/10.1117/1.2992140

Published: 1 September 2008

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KEYWORDS

Light scattering

Blood

Refractive index

Scattering

Optical spheres

Luminescence

Laser scattering

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