Identification of different bacterial species in biofilms using confocal Raman microscopy

Brooke D. Beier; Robert G. Quivey Jr.; Andrew J. Berger

doi:10.1117/1.3505010

1 November 2010 Identification of different bacterial species in biofilms using confocal Raman microscopy

Brooke D. Beier, Robert G. Quivey Jr., Andrew J. Berger

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Journal of Biomedical Optics, Vol. 15, Issue 6, 066001 (November 2010). https://doi.org/10.1117/1.3505010

Abstract

Confocal Raman microspectroscopy is used to discriminate between different species of bacteria grown in biofilms. Tests are performed using two bacterial species, Streptococcus sanguinis and Streptococcus mutans, which are major components of oral plaque and of particular interest due to their association with healthy and cariogenic plaque, respectively. Dehydrated biofilms of these species are studied as a simplified model of dental plaque. A prediction model based on principal component analysis and logistic regression is calibrated using pure biofilms of each species and validated on pure biofilms grown months later, achieving 96% accuracy in prospective classification. When biofilms of the two species are partially mixed together, Raman-based identifications are achieved within ~2 µm of the boundaries between species with 97% accuracy. This combination of spatial resolution and predication accuracy should be suitable for forming images of species distributions within intact two-species biofilms.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Brooke D. Beier, Robert G. Quivey Jr., and Andrew J. Berger "Identification of different bacterial species in biofilms using confocal Raman microscopy," Journal of Biomedical Optics 15(6), 066001 (1 November 2010). https://doi.org/10.1117/1.3505010

Published: 1 November 2010

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