Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration

Pu Wang; Ping Wang; Han-Wei Wang; Ji-Xin Cheng

doi:10.1117/1.JBO.17.9.096010

14 September 2012 Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration

Pu Wang, Ping Wang, Han-Wei Wang, Ji-Xin Cheng

Author Affiliations +

Journal of Biomedical Optics, Vol. 17, Issue 9, 096010 (September 2012). https://doi.org/10.1117/1.JBO.17.9.096010

Abstract

Photoacoustic microscopy using vibrational overtone absorption as a contrast mechanism allows bond-selective imaging of deep tissues. Due to the spectral similarity of molecules in the region of overtone vibration, it is difficult to interrogate chemical components using photoacoustic signal at single excitation wavelength. Here we demonstrate that lipids and collagen, two critical markers for many kinds of diseases, can be distinguished by multispectral photoacoustic imaging of the first overtone of C-H bond. A phantom consisting of rat-tail tendon and fat was constructed to demonstrate this technique. Wavelengths between 1650 and 1850 nm were scanned to excite both the first overtone and combination bands of C-H bonds. B-scan multispectral photoacoustic images, in which each pixel contains a spectrum, were analyzed by a multivariate curve resolution-alternating least squares algorithm to recover the spatial distribution of collagen and lipids in the phantom.

Citation Download Citation

Pu Wang, Ping Wang, Han-Wei Wang, and Ji-Xin Cheng "Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration," Journal of Biomedical Optics 17(9), 096010 (14 September 2012). https://doi.org/10.1117/1.JBO.17.9.096010

Published: 14 September 2012

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 58 scholarly publications and 1 patent.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Collagen

Tissues

Multispectral imaging

Photoacoustic spectroscopy

Photoacoustic imaging

Absorption

Chemical analysis

Show All Keywords

RELATED CONTENT

Photoacoustic physio chemical analysis and its implementation in deep tissue...
Proceedings of SPIE (March 11 2015)

High frequency photoacoustic microscopy of hematoxylin and eosin stained slides...
Proceedings of SPIE (December 19 2022)

Spectroscopic photoacoustics for assessing ischemic kidney damage
Proceedings of SPIE (February 19 2018)

Unsupervised deep learning approach for photoacoustic spectral unmixing
Proceedings of SPIE (February 17 2020)

An interventional multispectral photoacoustic imaging platform for the guidance of...
Proceedings of SPIE (July 16 2015)

Hyperspectral vibrational photoacoustic imaging of lipids and collagen
Proceedings of SPIE (February 23 2012)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years