Improved detectability of neuronal connectivity on mechanical sectioning setup by using confocal detection

Xiaoli Qi; Hanqing Xiong; Xiaohua Lv; Jianling Chen; Hui Gong; Qingming Luo; Shaoqun Zeng

doi:10.1117/1.JBO.18.5.050506

28 May 2013 Improved detectability of neuronal connectivity on mechanical sectioning setup by using confocal detection

Xiaoli Qi, Hanqing Xiong, Xiaohua Lv, Jianling Chen, Hui Gong, Qingming Luo, Shaoqun Zeng

Author Affiliations +

Journal of Biomedical Optics, Vol. 18, Issue 5, 050506 (May 2013). https://doi.org/10.1117/1.JBO.18.5.050506

Abstract

A recently reported micro-optical sectioning tomography system has great potential to draw the neuronal circuits of large brain volume with submicron resolution by combining fine mechanic sectioning with simultaneous optical imaging. However, sectioning the fluorescence sample sometimes induces tears between adjacent tiles and causes difficulties in continuous fiber tracing from fluorescence imaging. A confocal detection to recover the interruptions of the nerve fiber is introduced. With a 50-μm-width confocal slit, the signal-to-background ratio is increased 16- to 49-fold more than that without the slit, which effectively improves the detectability of the signal in the interruptions and enables continuous tracing of the neuronal circuits.

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Xiaoli Qi, Hanqing Xiong, Xiaohua Lv, Jianling Chen, Hui Gong, Qingming Luo, and Shaoqun Zeng "Improved detectability of neuronal connectivity on mechanical sectioning setup by using confocal detection," Journal of Biomedical Optics 18(5), 050506 (28 May 2013). https://doi.org/10.1117/1.JBO.18.5.050506

Published: 28 May 2013

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