Laser speckle contrast imaging of blood flow in rat retinas using an endoscope

Adrien Ponticorvo; Damon Cardenas; Andrew K. Dunn; Daniel Ts'o; Timothy Q. Duong

doi:10.1117/1.JBO.18.9.090501

24 September 2013 Laser speckle contrast imaging of blood flow in rat retinas using an endoscope

Adrien Ponticorvo, Damon Cardenas, Andrew K. Dunn, Daniel Ts'o, Timothy Q. Duong

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Journal of Biomedical Optics, Vol. 18, Issue 9, 090501 (September 2013). https://doi.org/10.1117/1.JBO.18.9.090501

Abstract

Laser speckle contrast imaging (LSCI) offers a cost-effective means to image blood flow in vivo. However, it is not commonly used to image rodent retinas because of the challenges associated with imaging through the curved cornea and delivering light through the highly scattering lens. A solution to overcome these problems by using LSCI in conjunction with an endoscope to obtain high spatiotemporal blood flow images is described. Its utility is demonstrated by imaging blood flow changes in rat retinas using hyperoxic, hypercapnic, and visual (flicker) stimulations. Hypercapnia increases blood flow, hyperoxia decreases blood flow, and visual stimulation increases blood flow in the retina relative to basal conditions. The time-to-peak of the LSCI response to visual stimulation is also measured. This approach may prove useful to investigate dysregulation in blood flow-evoked responses in retinal diseases and to evaluate treatment strategies in rodents.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Adrien Ponticorvo, Damon Cardenas, Andrew K. Dunn, Daniel Ts'o, and Timothy Q. Duong "Laser speckle contrast imaging of blood flow in rat retinas using an endoscope," Journal of Biomedical Optics 18(9), 090501 (24 September 2013). https://doi.org/10.1117/1.JBO.18.9.090501

Published: 24 September 2013

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