Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing

Piotr J. Cywiński; Tommy Hammann; Dominik Hühn; Wolfgang J. Parak; Niko Hildebrandt; Hans-Gerd Löhmannsröben

doi:10.1117/1.JBO.19.10.101506

2 July 2014 Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing

Piotr J. Cywiński, Tommy Hammann, Dominik Hühn, Wolfgang J. Parak, Niko Hildebrandt, Hans-Gerd Löhmannsröben

Author Affiliations +

Journal of Biomedical Optics, Vol. 19, Issue 10, 101506 (July 2014). https://doi.org/10.1117/1.JBO.19.10.101506

Abstract

Nanobioconjugates have been synthesized using cadmium selenide quantum dots (QDs), europium complexes (EuCs), and biotin. In those conjugates, long-lived photoluminescence (PL) is provided by the europium complexes, which efficiently transfer energy via Förster resonance energy transfer (FRET) to the QDs in close spatial proximity. As a result, the conjugates have a PL emission spectrum characteristic for QDs combined with the long PL decay time characteristic for EuCs. The nanobioconjugates synthesis strategy and photophysical properties are described as well as their performance in a time-resolved streptavidin-biotin PL assay. In order to prepare the QD-EuC-biotin conjugates, first an amphiphilic polymer has been functionalized with the EuC and biotin. Then, the polymer has been brought onto the surface of the QDs (either QD655 or QD705) to provide functionality and to make the QDs water dispersible. Due to a short distance between EuC and QD, an efficient FRET can be observed. Additionally, the QD-EuC-biotin conjugates’ functionality has been demonstrated in a PL assay yielding good signal discrimination, both from autofluorescence and directly excited QDs. These newly designed QD-EuC-biotin conjugates expand the class of highly sensitive tools for bioanalytical optical detection methods for diagnostic and imaging applications.

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Piotr J. Cywiński, Tommy Hammann, Dominik Hühn, Wolfgang J. Parak, Niko Hildebrandt, and Hans-Gerd Löhmannsröben "Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing," Journal of Biomedical Optics 19(10), 101506 (2 July 2014). https://doi.org/10.1117/1.JBO.19.10.101506

Published: 2 July 2014

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KEYWORDS

Fluorescence resonance energy transfer

Polymers

Europium

Luminescence

Ions

Sensors

Biosensing

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