Two-step phase-shifting fluorescence incoherent holographic microscopy

Wan Qin; Xiaoqi Yang; Yingying Li; Xiang Peng; Hai Yao; Xinghua Qu; Bruce Z. Gao

doi:10.1117/1.JBO.19.6.060503

27 June 2014 Two-step phase-shifting fluorescence incoherent holographic microscopy

Wan Qin, Xiaoqi Yang, Yingying Li, Xiang Peng, Hai Yao, Xinghua Qu, Bruce Z. Gao

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Journal of Biomedical Optics, Vol. 19, Issue 6, 060503 (June 2014). https://doi.org/10.1117/1.JBO.19.6.060503

Abstract

Fluorescence holographic microscope (FINCHSCOPE) is a motionless fluorescence holographic imaging technique based on Fresnel incoherent correlation holography (FINCH) that shows promise in reconstructing three-dimensional fluorescence images of biological specimens with three holograms. We report a developing two-step phase-shifting method that reduces the required number of holograms from three to two. Using this method, we resolved microscopic fluorescent beads that were three-dimensionally distributed at different depths with two interferograms captured by a CCD camera. The method enables the FINCHSCOPE to work in conjunction with the frame-straddling technique and significantly enhance imaging speed.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Wan Qin, Xiaoqi Yang, Yingying Li, Xiang Peng, Hai Yao, Xinghua Qu, and Bruce Z. Gao "Two-step phase-shifting fluorescence incoherent holographic microscopy," Journal of Biomedical Optics 19(6), 060503 (27 June 2014). https://doi.org/10.1117/1.JBO.19.6.060503

Published: 27 June 2014

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