Relationship between the v2PO4/amide III ratio assessed by Raman spectroscopy and the calcium content measured by quantitative backscattered electron microscopy in healthy human osteonal bone

Andreas Roschger; Sonja Gamsjaeger; Birgit Hofstetter; Admir Masic; Stéphane Blouin; Phaedra Messmer; Andrea Berzlanovich; Eleftherios P. Paschalis; Paul Roschger; Klaus Klaushofer; Peter Fratzl

doi:10.1117/1.JBO.19.6.065002

11 June 2014 Relationship between the v2PO4/amide III ratio assessed by Raman spectroscopy and the calcium content measured by quantitative backscattered electron microscopy in healthy human osteonal bone

Andreas Roschger, Sonja Gamsjaeger, Birgit Hofstetter, Admir Masic, Stéphane Blouin, Phaedra Messmer, Andrea Berzlanovich, Eleftherios P. Paschalis, Paul Roschger, Klaus Klaushofer, Peter Fratzl

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Journal of Biomedical Optics, Vol. 19, Issue 6, 065002 (June 2014). https://doi.org/10.1117/1.JBO.19.6.065002

Abstract

Raman microspectroscopy and quantitative backscattered electron imaging (qBEI) of bone are powerful tools to investigate bone material properties. Both methods provide information on the degree of bone matrix mineralization. However, a head-to-head comparison of these outcomes from identical bone areas has not been performed to date. In femoral midshaft cross sections of three women, 99 regions (20×20 μm 2 ) were selected inside osteons and interstitial bone covering a wide range of matrix mineralization. As the focus of this study was only on regions undergoing secondary mineralization, zones exhibiting a distinct gradient in mineral content close to the mineralization front were excluded. The same regions were measured by both methods. We found a linear correlation (R ² =0.75 ) between mineral/matrix as measured by Raman spectroscopy and the wt. %Mineral/(100-wt. %Mineral) as obtained by qBEI, in good agreement with theoretical estimations. The observed deviations of single values from the linear regression line were determined to reflect biological heterogeneities. The data of this study demonstrate the good correspondence between Raman and qBEI outcomes in describing tissue mineralization. The obtained correlation is likely sensitive to changes in bone tissue composition, providing an approach to detect potential deviations from normal bone.

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Andreas Roschger, Sonja Gamsjaeger, Birgit Hofstetter, Admir Masic, Stéphane Blouin, Phaedra Messmer, Andrea Berzlanovich, Eleftherios P. Paschalis, Paul Roschger, Klaus Klaushofer, and Peter Fratzl "Relationship between the v2PO4/amide III ratio assessed by Raman spectroscopy and the calcium content measured by quantitative backscattered electron microscopy in healthy human osteonal bone," Journal of Biomedical Optics 19(6), 065002 (11 June 2014). https://doi.org/10.1117/1.JBO.19.6.065002

Published: 11 June 2014

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