Investigating the influence of chromatic aberration and optical illumination bandwidth on fundus imaging in rats

Hao Li; Wenzhong Liu; Hao  F. Zhang

doi:10.1117/1.JBO.20.10.106010

23 October 2015 Investigating the influence of chromatic aberration and optical illumination bandwidth on fundus imaging in rats

Hao Li, Wenzhong Liu, Hao F. Zhang

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Journal of Biomedical Optics, Vol. 20, Issue 10, 106010 (October 2015). https://doi.org/10.1117/1.JBO.20.10.106010

Abstract

Rodent models are indispensable in studying various retinal diseases. Noninvasive, high-resolution retinal imaging of rodent models is highly desired for longitudinally investigating the pathogenesis and therapeutic strategies. However, due to severe aberrations, the retinal image quality in rodents can be much worse than that in humans. We numerically and experimentally investigated the influence of chromatic aberration and optical illumination bandwidth on retinal imaging. We confirmed that the rat retinal image quality decreased with increasing illumination bandwidth. We achieved the retinal image resolution of 10 μm using a 19 nm illumination bandwidth centered at 580 nm in a home-built fundus camera. Furthermore, we observed higher chromatic aberration in albino rat eyes than in pigmented rat eyes. This study provides a design guide for high-resolution fundus camera for rodents. Our method is also beneficial to dispersion compensation in multiwavelength retinal imaging applications.

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Hao Li, Wenzhong Liu, and Hao F. Zhang "Investigating the influence of chromatic aberration and optical illumination bandwidth on fundus imaging in rats," Journal of Biomedical Optics 20(10), 106010 (23 October 2015). https://doi.org/10.1117/1.JBO.20.10.106010

Published: 23 October 2015

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