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16 April 2015 Labeling of HeLa cells using ZrO2:Yb3+-Er3+ nanoparticles with upconversion emission
Andrea Ceja-Fdez, Tzarara López-Luke, Jorge Oliva, Juan Vivero-Escoto, Ana Lilia Gonzalez-Yebra, Ruben A. Rodríguez-Rojas, Andrea Martínez-Pérez, Elder de la Rosa
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Journal of Biomedical Optics, Vol. 20, Issue 4, 046006 (April 2015). https://doi.org/10.1117/1.JBO.20.4.046006
Abstract
This work reports the synthesis, structural characterization, and optical properties of ZrO2:Yb3+-Er3+ (2–1 mol%) nanocrystals. The nanoparticles were coated with 3-aminopropyl triethoxysilane (APTES) and further modified with biomolecules, such as Biotin-Anti-rabbit (mouse IgG) and rabbit antibody-AntiKi-67, through a conjugation method. The conjugation was successfully confirmed by Fourier transform infrared, zeta potential, and dynamic light scattering. The internalization of the conjugated nanoparticles in human cervical cancer (HeLa) cells was followed by two-photon confocal microscopy. The ZrO2:Yb3+-Er3+ nanocrystals exhibited strong red emission under 970-nm excitation. Moreover, the luminescence change due to the addition of APTES molecules and biomolecules on the nanocrystals was also studied. These results demonstrate that ZrO2:Yb3+-Er3+ nanocrystals can be successfully functionalized with biomolecules to develop platforms for biolabeling and bioimaging.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Andrea Ceja-Fdez, Tzarara López-Luke, Jorge Oliva, Juan Vivero-Escoto, Ana Lilia Gonzalez-Yebra, Ruben A. Rodríguez-Rojas, Andrea Martínez-Pérez, and Elder de la Rosa "Labeling of HeLa cells using ZrO2:Yb3+-Er3+ nanoparticles with upconversion emission," Journal of Biomedical Optics 20(4), 046006 (16 April 2015). https://doi.org/10.1117/1.JBO.20.4.046006
Published: 16 April 2015
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Cited by 13 scholarly publications.
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KEYWORDS
Ytterbium
Nanoparticles
Nanocrystals
Luminescence
FT-IR spectroscopy
Erbium
Cancer
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