Combination of low level light therapy and nitrosyl-cobinamide accelerates wound healing

Ryan Spitler; Hsiang Ho; Frederique Norpetlian; Xiangduo Kong; Jingjing Jiang; Kyoko Yokomori; Bogi Andersen; Gerry R. Boss M.D.; Michael W. Berns

doi:10.1117/1.JBO.20.5.051022

6 January 2015 Combination of low level light therapy and nitrosyl-cobinamide accelerates wound healing

Ryan Spitler, Hsiang Ho, Frederique Norpetlian, Xiangduo Kong, Jingjing Jiang, Kyoko Yokomori, Bogi Andersen, Gerry R. Boss M.D., Michael W. Berns

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Journal of Biomedical Optics, Vol. 20, Issue 5, 051022 (January 2015). https://doi.org/10.1117/1.JBO.20.5.051022

Abstract

Low level light therapy (LLLT) has numerous therapeutic benefits, including improving wound healing, but the precise mechanisms involved are not well established; in particular, the underlying role of cytochrome C oxidase (C-ox) as the primary photoacceptor and the associated biochemical mechanisms still require further investigation. We previously showed the nitric oxide (NO) donating drug nitrosyl-cobinamide (NO-Cbi) enhances wound healing through a cGMP/cGMP-dependent protein kinase/ERK1/2 mechanism. Here, we show that the combination of LLLT and NO-Cbi markedly improves wound healing compared to either treatment alone. LLLT-enhanced wound healing proceeded through an electron transport chain-C-ox-dependent mechanism with a reduction of reactive oxygen species and increased adenosine triphosphate production. C-ox was validated as the primary photoacceptor by three observations: increased oxygen consumption, reduced wound healing in the presence of sodium azide, and disassociation of cyanide, a known C-ox ligand, following LLLT. We conclude that LLLT and NO-Cbi accelerate wound healing through two independent mechanisms, the electron transport chain-C-ox pathway and cGMP signaling, respectively, with both resulting in ERK1/2 activation.

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Ryan Spitler, Hsiang Ho, Frederique Norpetlian, Xiangduo Kong, Jingjing Jiang, Kyoko Yokomori, Bogi Andersen, Gerry R. Boss M.D., and Michael W. Berns "Combination of low level light therapy and nitrosyl-cobinamide accelerates wound healing," Journal of Biomedical Optics 20(5), 051022 (6 January 2015). https://doi.org/10.1117/1.JBO.20.5.051022

Published: 6 January 2015

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