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11 August 2015 Raman spectroscopy for label-free identification of calciphylaxis
William R. Lloyd, Shailesh Agarwal, Sagar U. Nigwekar, Shawn Fagan, Jeremy Goverman, Karen Esmonde-White, Shawn Loder, Bjorn R. Olsen, Dolrudee Jumlongras, Michael D. Morris, Benjamin Levi
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Journal of Biomedical Optics, Vol. 20, Issue 8, 080501 (August 2015). https://doi.org/10.1117/1.JBO.20.8.080501
Abstract
Calciphylaxis is a painful, debilitating, and premorbid condition, which presents as calcified vasculature and soft tissues. Traditional diagnosis of calciphylaxis lesions requires an invasive biopsy, which is destructive, time consuming, and often leads to exacerbation of the condition and infection. Furthermore, it is difficult to find small calcifications within a large wound bed. To address this need, a noninvasive diagnostic tool may help clinicians identify ectopic calcified mineral and determine the disease margin. We propose Raman spectroscopy as a rapid, point-of-care, noninvasive, and label-free technology to detect calciphylaxis mineral. Debrided calciphylactic tissue was collected from six patients and assessed by microcomputed tomography (micro-CT). Micro-CT confirmed extensive deposits in three specimens, which were subsequently examined with Raman spectroscopy. Raman spectra confirmed that deposits were consistent with carbonated apatite, consistent with the literature. Raman spectroscopy shows potential as a noninvasive technique to detect calciphylaxis in a clinical environment.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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William R. Lloyd, Shailesh Agarwal, Sagar U. Nigwekar, Shawn Fagan, Jeremy Goverman, Karen Esmonde-White, Shawn Loder, Bjorn R. Olsen, Dolrudee Jumlongras, Michael D. Morris, and Benjamin Levi "Raman spectroscopy for label-free identification of calciphylaxis," Journal of Biomedical Optics 20(8), 080501 (11 August 2015). https://doi.org/10.1117/1.JBO.20.8.080501
Published: 11 August 2015
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Cited by 11 scholarly publications.
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KEYWORDS
Raman spectroscopy
Tissues
Surgery
Apatite
Biopsy
Diagnostics
Minerals
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