Feasibility study: protein denaturation and coagulation monitoring with speckle variance optical coherence tomography

Changho Lee; Gyeongwoo Cheon; Do-Hyun Kim; Jin U. Kang

doi:10.1117/1.JBO.21.12.125004

10 December 2016 Feasibility study: protein denaturation and coagulation monitoring with speckle variance optical coherence tomography

Changho Lee, Gyeongwoo Cheon, Do-Hyun Kim, Jin U. Kang

Author Affiliations +

Journal of Biomedical Optics, Vol. 21, Issue 12, 125004 (December 2016). https://doi.org/10.1117/1.JBO.21.12.125004

Abstract

We performed the feasibility study using speckle variance optical coherence tomography (SvOCT) to monitor the thermally induced protein denaturation and coagulation process as a function of temperature and depth. SvOCT provided the depth-resolved image of protein denaturation and coagulation with microscale resolution. This study was conducted using egg white. During the heating process, as the temperature increased, increases in the speckle variance signal was observed as the egg white proteins coagulated. Additionally, by calculating the cross-correlation coefficient in specific areas, denaturized egg white conditions were successfully estimated. These results indicate that SvOCT could be used to monitor the denaturation process of various proteins.

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Changho Lee, Gyeongwoo Cheon, Do-Hyun Kim, and Jin U. Kang "Feasibility study: protein denaturation and coagulation monitoring with speckle variance optical coherence tomography," Journal of Biomedical Optics 21(12), 125004 (10 December 2016). https://doi.org/10.1117/1.JBO.21.12.125004

Published: 10 December 2016

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KEYWORDS

Optical coherence tomography

Speckle

Proteins

Temperature metrology

Tissues

Aluminum

Finite-difference time-domain method

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