Multimodal computational microscopy based on transport of intensity equation

Jiaji Li; Qian Chen; Jiasong Sun; Jialin Zhang; Chao Zuo

doi:10.1117/1.JBO.21.12.126003

5 December 2016 Multimodal computational microscopy based on transport of intensity equation

Jiaji Li, Qian Chen, Jiasong Sun, Jialin Zhang, Chao Zuo

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Journal of Biomedical Optics, Vol. 21, Issue 12, 126003 (December 2016). https://doi.org/10.1117/1.JBO.21.12.126003

Abstract

Transport of intensity equation (TIE) is a powerful tool for phase retrieval and quantitative phase imaging, which requires intensity measurements only at axially closely spaced planes without a separate reference beam. It does not require coherent illumination and works well on conventional bright-field microscopes. The quantitative phase reconstructed by TIE gives valuable information that has been encoded in the complex wave field by passage through a sample of interest. Such information may provide tremendous flexibility to emulate various microscopy modalities computationally without requiring specialized hardware components. We develop a requisite theory to describe such a hybrid computational multimodal imaging system, which yields quantitative phase, Zernike phase contrast, differential interference contrast, and light field moment imaging, simultaneously. It makes the various observations for biomedical samples easy. Then we give the experimental demonstration of these ideas by time-lapse imaging of live HeLa cell mitosis. Experimental results verify that a tunable lens-based TIE system, combined with the appropriate postprocessing algorithm, can achieve a variety of promising imaging modalities in parallel with the quantitative phase images for the dynamic study of cellular processes.

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Jiaji Li, Qian Chen, Jiasong Sun, Jialin Zhang, and Chao Zuo "Multimodal computational microscopy based on transport of intensity equation," Journal of Biomedical Optics 21(12), 126003 (5 December 2016). https://doi.org/10.1117/1.JBO.21.12.126003

Published: 5 December 2016

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