Protease sensing using nontoxic silicon quantum dots

Xiaoyu Cheng; Benjamin F. P. McVey; Andrew B. Robinson; Guillaume Longatte; Peter B. O’Mara; Vincent T. G. Tan; Pall Thordarson; Richard D. Tilley; Katharina Gaus; J. Justin Gooding

doi:10.1117/1.JBO.22.8.087002

23 August 2017 Protease sensing using nontoxic silicon quantum dots

Xiaoyu Cheng, Benjamin F. P. McVey, Andrew B. Robinson, Guillaume Longatte, Peter B. O’Mara, Vincent T. G. Tan, Pall Thordarson, Richard D. Tilley, Katharina Gaus, J. Justin Gooding

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Journal of Biomedical Optics, Vol. 22, Issue 8, 087002 (August 2017). https://doi.org/10.1117/1.JBO.22.8.087002

Abstract

Herein is presented a proof-of-concept study of protease sensing that combines nontoxic silicon quantum dots (SiQDs) with Förster resonance energy transfer (FRET). The SiQDs serve as the donor and an organic dye as the acceptor. The dye is covalently attached to the SiQDs using a peptide linker. Enzymatic cleavage of the peptide leads to changes in FRET efficiency. The combination of interfacial design and optical imaging presented in this work opens opportunities for use of nontoxic SiQDs relevant to intracellular sensing and imaging.

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Xiaoyu Cheng, Benjamin F. P. McVey, Andrew B. Robinson, Guillaume Longatte, Peter B. O’Mara, Vincent T. G. Tan, Pall Thordarson, Richard D. Tilley, Katharina Gaus, and J. Justin Gooding "Protease sensing using nontoxic silicon quantum dots," Journal of Biomedical Optics 22(8), 087002 (23 August 2017). https://doi.org/10.1117/1.JBO.22.8.087002

Received: 25 April 2017; Accepted: 31 July 2017; Published: 23 August 2017

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