Modulus design multiwavelength polarization microscope for transmission Mueller matrix imaging

Jialing Zhou; Honghui He; Zhenhua Chen; Ye Wang; Hui Ma

doi:10.1117/1.JBO.23.1.016007

8 January 2018 Modulus design multiwavelength polarization microscope for transmission Mueller matrix imaging

Jialing Zhou, Honghui He, Zhenhua Chen, Ye Wang, Hui Ma

Author Affiliations +

Funded by: National Natural Science Foundation of China (NSFC), National Natural Science Foundation of China, Natural Science Foundation of China, Science and Technology Project of Shenzhen, Science Technology Project of Shenzhen

Journal of Biomedical Optics, Vol. 23, Issue 1, 016007 (January 2018). https://doi.org/10.1117/1.JBO.23.1.016007

Abstract

We have developed a polarization microscope based on a commercial transmission microscope. We replace the halogen light source by a collimated LED light source module of six different colors. We use achromatic polarized optical elements that can cover the six different wavelength ranges in the polarization state generator (PSG) and polarization state analyzer (PSA) modules. The dual-rotating wave plate method is used to measure the Mueller matrix of samples, which requires the simultaneous rotation of the two quarter-wave plates in both PSG and PSA at certain angular steps. A scientific CCD detector is used as the image receiving module. A LabView-based software is developed to control the rotation angels of the wave plates and the exposure time of the detector to allow the system to run fully automatically in preprogrammed schedules. Standard samples, such as air, polarizers, and quarter-wave plates, are used to calibrate the intrinsic Mueller matrix of optical components, such as the objectives, using the eigenvalue calibration method. Errors due to the images walk-off in the PSA are studied. Errors in the Mueller matrices are below 0.01 using air and polarizer as standard samples. Data analysis based on Mueller matrix transformation and Mueller matrix polarization decomposition is used to demonstrate the potential application of this microscope in pathological diagnosis.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Jialing Zhou, Honghui He, Zhenhua Chen, Ye Wang, and Hui Ma "Modulus design multiwavelength polarization microscope for transmission Mueller matrix imaging," Journal of Biomedical Optics 23(1), 016007 (8 January 2018). https://doi.org/10.1117/1.JBO.23.1.016007

Received: 1 October 2017; Accepted: 14 December 2017; Published: 8 January 2018

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