Polarization-sensitive optical coherence tomography imaging of the anterior mouse eye

Bernhard Baumann; Marco Augustin; Antonia Lichtenegger; Danielle J. Harper; Martina Muck; Pablo Eugui; Andreas Wartak; Michael Pircher; Christoph K. Hitzenberger

doi:10.1117/1.JBO.23.8.086005

30 August 2018 Polarization-sensitive optical coherence tomography imaging of the anterior mouse eye

Bernhard Baumann, Marco Augustin, Antonia Lichtenegger, Danielle J. Harper, Martina Muck, Pablo Eugui, Andreas Wartak, Michael Pircher, Christoph K. Hitzenberger

Author Affiliations +

Journal of Biomedical Optics, Vol. 23, Issue 8, 086005 (August 2018). https://doi.org/10.1117/1.JBO.23.8.086005

Abstract

Polarization-sensitive optical coherence tomography (PS-OCT) enables noninvasive, high-resolution imaging of tissue polarization properties. In the anterior segments of human eyes, PS-OCT allows the visualization of birefringent and depolarizing structures. We present the use of PS-OCT for imaging the murine anterior eye. Using a spectral domain PS-OCT setup operating in the 840-nm regime, we performed in vivo volumetric imaging in anesthetized C57BL/6 mice. The polarization properties of murine anterior eye structures largely replicated those known from human PS-OCT imagery, suggesting that the mouse eye may also serve as a model system under polarization contrast. However, dissimilarities were found in the depolarizing structure of the iris which, as we confirmed in postmortem histological sections, were caused by anatomical differences between both species. In addition to the imaging of tissues in the anterior chamber and the iridocorneal angle, we demonstrate longitudinal PS-OCT imaging of the murine anterior segment during mydriasis as well as birefringence imaging of corneal pathology in an aged mouse.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Bernhard Baumann, Marco Augustin, Antonia Lichtenegger, Danielle J. Harper, Martina Muck, Pablo Eugui, Andreas Wartak, Michael Pircher, and Christoph K. Hitzenberger "Polarization-sensitive optical coherence tomography imaging of the anterior mouse eye," Journal of Biomedical Optics 23(8), 086005 (30 August 2018). https://doi.org/10.1117/1.JBO.23.8.086005

Received: 5 June 2018; Accepted: 14 August 2018; Published: 30 August 2018

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