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10 September 2018 Photobiomodulation therapy improves multilineage differentiation of dental pulp stem cells in three-dimensional culture model
Ivana Maria Zaccara, Letícia Boldrin Mestieri, Maria Stella Moreira, Fabiana Soares Grecca, Manoela Domingues Martins, Patrícia Maria Poli Kopper
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Journal of Biomedical Optics, Vol. 23, Issue 9, 095001 (September 2018). https://doi.org/10.1117/1.JBO.23.9.095001
Abstract
Photobiomodulation therapy (PBM) has shown positive effects on stem cell differentiation in monolayer cell culture model, but little is known about its effect on three-dimensional (3-D) agarose gel culture. This study evaluated the PBM effect of human dental pulp stem cells (hDPSCs) differentiation and phosphatase alkaline activity (ALP) using an agarose 3-D model under different nutritional conditions. hDPSCs were characterized and seeded on a 0.3% agarose gel layer with different media (osteogenic, adipogenic, chondrogenic) and were assigned into four groups: control 10% fetal bovine serum (FBS), control 5% FBS, PBM 10% FBS, and PBM 5% FBS. Irradiation was performed with continuous-wave InGaAlP laser, 660 nm, 100 mW, 3,3  J  /  cm2, spot size 0.3  cm2, 10 s of exposure time, and 1 J of energy per point with 6-h interval between sessions. All groups were evaluated at 7 and 14 days. ALP assay was performed to analyze the deposition of mineralized tissue. At 7 days, PBM 5% FBS group presented better stimulation in osteogenic and adipogenic differentiation compared with control. After 14 days, hDPSCs cultured in 3-D exhibited osteogenic, adipogenic, and chondrogenic differentiation; furthermore, compared to control, PBM significantly stimulated all differentiation processes (p  <  0.05). It can be concluded that hDPSCs cultured in 3-D agarose associated to PBM could be a promising tool for tissue engineering applications.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2018/$25.00 © 2018 SPIE
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Ivana Maria Zaccara, Letícia Boldrin Mestieri, Maria Stella Moreira, Fabiana Soares Grecca, Manoela Domingues Martins, and Patrícia Maria Poli Kopper "Photobiomodulation therapy improves multilineage differentiation of dental pulp stem cells in three-dimensional culture model," Journal of Biomedical Optics 23(9), 095001 (10 September 2018). https://doi.org/10.1117/1.JBO.23.9.095001
Received: 22 March 2018; Accepted: 17 August 2018; Published: 10 September 2018
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Cited by 12 scholarly publications and 1 patent.
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KEYWORDS
3D modeling
Stem cells
Aluminium phosphide
Tissues
Laser therapeutics
Laser tissue interaction
Microelectromechanical systems
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