Validation of optical properties quantification with a dual-step technique for biological tissue analysis

Verónica Sorgato; Michel Berger; Charlotte Emain; Christine Vever-Bizet; Jean-Marc Dinten; Geneviève Bourg-Heckly; Anne Planat-Chrétien

doi:10.1117/1.JBO.23.9.096002

19 September 2018 Validation of optical properties quantification with a dual-step technique for biological tissue analysis

Verónica Sorgato, Michel Berger, Charlotte Emain, Christine Vever-Bizet, Jean-Marc Dinten, Geneviève Bourg-Heckly, Anne Planat-Chrétien

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Journal of Biomedical Optics, Vol. 23, Issue 9, 096002 (September 2018). https://doi.org/10.1117/1.JBO.23.9.096002

Abstract

To approach wide-field optical properties quantification in real heterogeneous biological tissue, we developed a Dual-Step setup that couples a punctual diffuse reflectance spectroscopy (DRS) technique with multispectral imaging (MSI). The setup achieves wide-field optical properties assessment through an initial estimation of scattering with DRS, which is used to estimate absorption with MSI. The absolute quantification of optical properties is based on the ACA-Pro algorithm that has been adapted both for DRS and for MSI. This paper validates the Dual-Step system not only on homogeneous Intralipid phantoms but also on a heterogeneous gelatine phantom with different scattering and absorbing properties.

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Verónica Sorgato, Michel Berger, Charlotte Emain, Christine Vever-Bizet, Jean-Marc Dinten, Geneviève Bourg-Heckly, and Anne Planat-Chrétien "Validation of optical properties quantification with a dual-step technique for biological tissue analysis," Journal of Biomedical Optics 23(9), 096002 (19 September 2018). https://doi.org/10.1117/1.JBO.23.9.096002

Received: 30 August 2017; Accepted: 6 August 2018; Published: 19 September 2018

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