CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 5 > Issue 1 > Article
Open Access
1 January 2000 Blood plasma coagulation studied by surface plasmon resonance
Trine P. Vikinge, Kenny M. Hansson, Johan Benesch, Knut Johansen, Mats Ranby, Tomas L. Lindahl, Bo Liedberg, Ingemar Lundstoem, Pentti Tengvall
Author Affiliations +
Journal of Biomedical Optics, Vol. 5, Issue 1, (January 2000). https://doi.org/10.1117/1.429968
A surface plasmon resonance (SPR) apparatus was used to investigate blood plasma coagulation in real time as a function of thromboplastin and heparin concentrations. The response curves were analyzed by curve fitting to a sigmoid curve equation, followed by extraction of the time constant. Clotting activation by thromboplastin resulted in increased time constant, as compared to spontaneously clotted plasma, in a dose dependent way. Addition of heparin to the thromboplastin-activated plasma counteracted this effect. Atomic force microscopy (AFM) pictures of sensor surfaces dried after completed clotting, revealed differences in fibrin network structures as a function of thromboplastin concentration, and the fiber thickness increased with decreased thromboplastin concentration. The physical reason for the SPR signal observed is ambiguous and is therefore discussed. However, the results summarized in the plots and the fibrin network properties observed by AFM correlate well with present common methods used to analyze blood coagulation.
Citation Download Citation
Trine P. Vikinge, Kenny M. Hansson, Johan Benesch, Knut Johansen, Mats Ranby, Tomas L. Lindahl, Bo Liedberg, Ingemar Lundstoem, and Pentti Tengvall "Blood plasma coagulation studied by surface plasmon resonance," Journal of Biomedical Optics 5(1), (1 January 2000). https://doi.org/10.1117/1.429968
Published: 1 January 2000
JOURNAL ARTICLE
 5 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 40 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Plasma
Blood
Sensors
Polymerization
Atomic force microscopy
Surface plasmons
Gold
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top