Two-photon excited fluorescence properties of glycation-associated collagen crosslinks that are correlated with tissue stiffness (Conference Presentation)

Xi Yu; Christopher Flynn; Isaac Lasko; Sauro Liberatore; Luis Dorfmann; Irene Georgakoudi

doi:10.1117/12.2507617

4 March 2019 Two-photon excited fluorescence properties of glycation-associated collagen crosslinks that are correlated with tissue stiffness (Conference Presentation)

Xi Yu, Christopher Flynn, Isaac Lasko, Sauro Liberatore, Luis Dorfmann, Irene Georgakoudi

Author Affiliations +

Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 108901E (2019) https://doi.org/10.1117/12.2507617
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Collagen is a main constituent of the extracellular matrix, and its content, organization, and crosslinking status affect significantly tissue mechanical properties. Cells continuously sense their local mechanical environment via interactions that impact significantly cell fate and the development and progression of numerous diseases. Non-linear imaging, especially through the combined use of second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), has the potential to provide detailed information regarding collagen organization, content, and crosslinking with sub-micron level resolution in a non-invasive and label-free manner. However, the crosslink fluorescence properties that are correlated with tissue stiffness aren’t well understood. Thus, we characterized the TPEF emission over 680 to 920 nm excitation and 400 to 780 nm emission of three types of collagen gels: a) plain collagen gels, b) collagen gels with a 250 mM ribose solution for five days prior to gelation, and c) following gelation. Uniaxial tensile testing of gels created using the same protocols was performed by a custom-built testing system. We identified two components, which peak at 440 nm and 510 nm, that were significantly correlated with the exponential stiffening detected in collagen gels treated with ribose following gelation. Thus, TPEF information acquired at these emission ranges can be used in future studies and in combination with SHG measurements to develop detailed mathematical models that aim to predict tissue micromechanical properties. The latter will be useful in helping us to understand mechanosensitive cell behaviors that ultimately dictate the progress of diseases such as fibrosis and cancer.

Conference Presentation

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Xi Yu, Christopher Flynn, Isaac Lasko, Sauro Liberatore, Luis Dorfmann, and Irene Georgakoudi "Two-photon excited fluorescence properties of glycation-associated collagen crosslinks that are correlated with tissue stiffness (Conference Presentation)", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108901E (4 March 2019); https://doi.org/10.1117/12.2507617

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KEYWORDS

Collagen

Tissues

Luminescence

Second-harmonic generation

Cancer

Environmental sensing

Harmonic generation

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