Quantitative analysis of collagen change between normal and cancerous thyroid tissues based on SHG method

Xiwen Chen; Zufang Huang; Gangqin Xi; Yongjian Chen; Duo Lin; Jing Wang; Zuanfang Li; Liqing Sun; Jianxin Chen; Rong Chen

doi:10.1117/12.918850

13 March 2012 Quantitative analysis of collagen change between normal and cancerous thyroid tissues based on SHG method

Xiwen Chen, Zufang Huang, Gangqin Xi, Yongjian Chen, Duo Lin, Jing Wang, Zuanfang Li, Liqing Sun, Jianxin Chen, Rong Chen

Author Affiliations +

Proceedings Volume 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011); 83290H (2012) https://doi.org/10.1117/12.918850
Event: Photonics and Optoelectronics Meetings 2011, 2011, Wuhan, China

Abstract

Second-harmonic generation (SHG) is proved to be a high spatial resolution, large penetration depth and non-photobleaching method. In our study, SHG method was used to investigate the normal and cancerous thyroid tissue. For SHG imaging performance, system parameters were adjusted for high-contrast images acquisition. Each x-y image was recorded in pseudo-color, which matches the wavelength range in the visible spectrum. The acquisition time for a 512×512-pixels image was 1.57 sec; each acquired image was averaged four frames to improve the signal-to-noise ratio. Our results indicated that collagen presence as determined by counting the ratio of the SHG pixels over the whole pixels for normal and cancerous thyroid tissues were 0.48±0.05, 0.33±0.06 respectively. In addition, to quantitatively assess collagen-related changes, we employed GLCM texture analysis to the SHG images. Corresponding results showed that the correlation both fell off with distance in normal and cancerous group. Calculated value of Corr₅₀ (the distance where the correlation crossed 50% of the initial correlation) indicated significant difference. This study demonstrates that SHG method can be used as a complementary tool in thyroid histopathology.

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Xiwen Chen, Zufang Huang, Gangqin Xi, Yongjian Chen, Duo Lin, Jing Wang, Zuanfang Li, Liqing Sun, Jianxin Chen, and Rong Chen "Quantitative analysis of collagen change between normal and cancerous thyroid tissues based on SHG method", Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290H (13 March 2012); https://doi.org/10.1117/12.918850

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