D-FFOCT as a tool to assess oxygenation induced changes in cellular metabolism (Conference Presentation)

Siddharth Khare; Ravi Malpani; Hyesoo Lee; Viswanath Gorti; Kosar Khaksari; Jules Scholler; Emilie Benoit; Claude Boccara; Dan Sackett; Amir Gandjbakhche

doi:10.1117/12.2549096

9 March 2020 D-FFOCT as a tool to assess oxygenation induced changes in cellular metabolism (Conference Presentation)

Siddharth Khare, Ravi Malpani, Hyesoo Lee, Viswanath Gorti, Kosar Khaksari, Jules Scholler, Emilie Benoit, Claude Boccara, Dan Sackett, Amir Gandjbakhche

Author Affiliations +

Proceedings Volume 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV; 112281R (2020) https://doi.org/10.1117/12.2549096
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Dynamic Full-Field Optical Coherence Tomography (D-FFOCT) is a high transverse resolution version of OCT that records signal at the output of the interferometer as a movie over a few seconds. Analyzing the temporal variation of the signals reveals intracellular contrast which gives D-FFOCT the capability of identifying dynamic metabolic changes. We use D-FFOCT to identify oxygen induced changes in cellular metabolism at ambient (22%) oxygenation and at hypoxia (1%). Signal strength, calculated as an integral of the raw FFT spectrum, is significantly higher for 1% samples compared to 22% indicating that D-FFOCT is sensitive to changes in cell metabolism.

Conference Presentation

Citation Download Citation

Siddharth Khare, Ravi Malpani, Hyesoo Lee, Viswanath Gorti, Kosar Khaksari, Jules Scholler, Emilie Benoit, Claude Boccara, Dan Sackett, and Amir Gandjbakhche "D-FFOCT as a tool to assess oxygenation induced changes in cellular metabolism (Conference Presentation)", Proc. SPIE 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, 112281R (9 March 2020); https://doi.org/10.1117/12.2549096

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