Rapid tumor margin analysis using paired-agent imaging to guide Mohs micrographic surgery

Veronica C. Torres; Sassan Hodge; Eunice Y. Chen; Joshua Levy; Louis J. Vaickus; Matthew LeBoeuf; Kimberley S. Samkoe

doi:10.1117/12.2609731

4 March 2022 Rapid tumor margin analysis using paired-agent imaging to guide Mohs micrographic surgery

Veronica C. Torres, Sassan Hodge, Eunice Y. Chen, Joshua Levy, Louis J. Vaickus, Matthew LeBoeuf, Kimberley S. Samkoe

Proceedings Volume 11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII; 1194304 (2022) https://doi.org/10.1117/12.2609731
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Thorough tumor resection is crucial for successful treatment of squamous cell carcinomas (SCCs) because positive surgical margins are associated with poor patient prognosis. Current methods of margin analysis, however, are limited by inefficient pathological read-times that increase exponentially with tissue size. Here, a fluorescence paired-agent imaging (PAI) approach is presented to identify regions of tumor burden in whole, thick tissue margins to act as a rapid screening tool and help focus pathological evaluation. The approach was applied to mouse models of head and neck SCC, and positive tumor burden was detected and localized in deep tissue margins up to 1.3 mm thick. Serial sections with hematoxylin and eosin and EGFR-immunostaining demonstrated good correlation with binding potential (BP: proportional to targeted biomolecule concentration) maps generated from PAI fluorescence slices and confirmed the presence of positive margins suggested by high intensity regions in the whole tissue BP maps. Findings support the use of PAI as a rapid screening method for detecting regions of tumor burden in large, en face tumor margin sections.

Conference Presentation

Citation Download Citation

Veronica C. Torres, Sassan Hodge, Eunice Y. Chen, Joshua Levy, Louis J. Vaickus, Matthew LeBoeuf, and Kimberley S. Samkoe "Rapid tumor margin analysis using paired-agent imaging to guide Mohs micrographic surgery", Proc. SPIE 11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII, 1194304 (4 March 2022); https://doi.org/10.1117/12.2609731

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