Optical coherence tomography guided Brillouin microscopy to study early embryonic development

Yogeshwari S. Ambekar; Manmohan Singh; Alexander W. Schill; Jitao Zhang; Christian Zevallos-Delgado; Behzad Khajavi; Salavat R. Aglyamov; Richard H. Finnell; Giuliano Scarcelli; Kirill V. Larin

doi:10.1117/12.2624346

27 May 2022 Optical coherence tomography guided Brillouin microscopy to study early embryonic development

Yogeshwari S. Ambekar, Manmohan Singh, Alexander W. Schill, Jitao Zhang, Christian Zevallos-Delgado, Behzad Khajavi, Salavat R. Aglyamov, Richard H. Finnell, Giuliano Scarcelli, Kirill V. Larin

Author Affiliations +

Proceedings Volume 12144, Biomedical Spectroscopy, Microscopy, and Imaging II; 121440H (2022) https://doi.org/10.1117/12.2624346
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

The mechanisms involved in neural tube formation are complex and can be easily disrupted. Neurulation is one such process, governed by mechanical forces where tissues physically fold and fuse. When neural tube folding and closure fail to complete during neurulation, it results in structural and functional abnormalities of the brain and spinal cord. Thus, it is important to understand the interplay between forces and tissue stiffness during neurulation. Brillouin microscopy is an all-optical, noninvasive, high-resolution imaging technique capable of mapping tissue stiffness, but it cannot provide structural information, resulting in “blind” imaging. To overcome this limitation, we have combined a Brillouin microscopy system with optical coherence tomography (OCT) in one synchronized and co-aligned instrument to provide structural guidance when mapping the biomechanical properties of neural tube formation in mouse embryos. We developed custom instrumentation control software that utilizes the OCT structural image to guide Brillouin imaging. We acquired first 3D OCT images and then 2D structural and mechanical maps of mouse embryos at embryonic day (E) 8.5, 9.5, and 10.5. Brillouin microscopy showed the cell-dense layer of neural plate derived from the ectoderm at E 8.5, which was unable to be distinguished with OCT. At E 9.5 and 10.5, the neuroepithelium could be clearly seen by Brillouin microscopy with a greater stiffness than the surrounding tissue. Our results show the capability of the co-aligned and synchronized Brillouin-OCT system to map tissue stiffness of murine embryos using OCT-guided Brillouin microscopy.

Conference Presentation

Citation Download Citation

Yogeshwari S. Ambekar, Manmohan Singh, Alexander W. Schill, Jitao Zhang, Christian Zevallos-Delgado, Behzad Khajavi, Salavat R. Aglyamov, Richard H. Finnell, Giuliano Scarcelli, and Kirill V. Larin "Optical coherence tomography guided Brillouin microscopy to study early embryonic development", Proc. SPIE 12144, Biomedical Spectroscopy, Microscopy, and Imaging II, 121440H (27 May 2022); https://doi.org/10.1117/12.2624346

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available