Micro-Raman spectroscopy of optically trapped single cell

Anpei Ye; Yong Zhang

doi:10.1117/12.838232

1 December 2009 Micro-Raman spectroscopy of optically trapped single cell

Anpei Ye, Yong Zhang

Proceedings Volume 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging; 750704 (2009) https://doi.org/10.1117/12.838232
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

We described and characterized an experimental system for micro-Raman spectroscopy of individual floating-particle or living cell trapped by single beam gradient optical trap (optical tweezers). This system combined a micro-Raman spectroscopy and optical tweezers technique, equipped an IR laser and another visible laser as trapping and Raman excitation beams, respectively. The Raman spectrum of floating-cell trapped by optical trap in liquid media has the advantage of eliminating the interference of cover-slips and confining cell Brownian motion. Moreover, using independent lasers enables optimizing the laser parameters for separately purpose, and modulating the Raman exciting beam position relative to trapping beam, also it is necessary for Raman imaging. Applying this system the Raman spectra of single living rat erythrocyte and saccharomyces cells was obtained with high spatial resolution. The results showed that this approach significantly improved the signal-to-noise ratio of Raman spectra of living cell compared with conventional way that immobilizes the floating-cell on the surface of cover-slips. This technique would provide a wide useful approach for the Raman spectroscopy of suspended micro-objects in aqueous solution, especial for single floatingcells.

Citation Download Citation

Anpei Ye and Yong Zhang "Micro-Raman spectroscopy of optically trapped single cell", Proc. SPIE 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging, 750704 (1 December 2009); https://doi.org/10.1117/12.838232

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