Application of the laser scanning confocal microscope in fluorescent film sensor research

Hongyan Zhang; Wei-Min Liu

doi:10.1117/12.865797

11 October 2010 Application of the laser scanning confocal microscope in fluorescent film sensor research

Hongyan Zhang, Wei-Min Liu

Proceedings Volume 7656, 5th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment; 76560Y (2010) https://doi.org/10.1117/12.865797
Event: 5th International Symposium on Advanced Optical Manufacturing and Testing Technologies, 2010, Dalian, China

Abstract

Confocal microscopy offers several advantages over conventional optical microscopy; we show an experimental investigation laser scanning confocal microscope as a tool to be used in cubic boron nitride (cBN) film-based fluorescent sensor research. Cubic boron nitride cBN film sensors are modified with dansyl chloride and rhodamine B isothiocyanate respectively. Fluorescent modification quality on the cubic boron nitride film is clearly express and the sensor ability to Hg2+ cations and pH are investigated in detail. We evidence the rhodamine B isothiocyanate modified quality on cBN surface is much better than that of dansyl chloride. And laser scanning confocal microscope has potential application lighttight fundus film fluorescent sensor research.

Citation Download Citation

Hongyan Zhang and Wei-Min Liu "Application of the laser scanning confocal microscope in fluorescent film sensor research", Proc. SPIE 7656, 5th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment, 76560Y (11 October 2010); https://doi.org/10.1117/12.865797

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