Association phenothiazine and laser on growth of C. tropicalis fluconazole-resistant

Rafael Araújo Gomes Júnior; Susana C. P. S. de Oliveira; Juliana S. C. Monteiro; Gustavo M. P. Santos; Fernando J. P. Sampaio D.D.S.; Maria F. M. Gesteira D.D.S.; Fátima A. A. Zanin; Aldo Brugnera Jr.; Antônio Luiz B. Pinheiro; Marcos A. Vannier-Santos

doi:10.1117/12.2038566

18 February 2014 Association phenothiazine and laser on growth of C. tropicalis fluconazole-resistant

Rafael Araújo Gomes Júnior, Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Gustavo M. P. Santos, Fernando J. P. Sampaio D.D.S., Maria F. M. Gesteira D.D.S., Fátima A. A. Zanin, Aldo Brugnera Jr., Antônio Luiz B. Pinheiro, Marcos A. Vannier-Santos

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Proceedings Volume 8932, Mechanisms for Low-Light Therapy IX; 89320G (2014) https://doi.org/10.1117/12.2038566
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Candidiasis is caused by Candida species found on the skin, gastrointestinal tract and mucous cavities of the humans and may be acute, chronic, localized or systemic. Alhough C. albicans is the species most often identified as responsible for this type of infection C. Tropicalis has been considered an emerging cause. The effect of the association of phenothiazine - PTZ and laser on fluconazole-resistant C.tropicalis growth was tested. 2.5 x 10⁶ CFU/mL 100mg/mL of phenothiazine with the pre-irradiation time of 10 min were irradiated with laser light (660 nm; 4.8 and 12 J/cm² (L1 and L2 respectively) 40 mW) followed by incubation in RPMI for 24h. The following conditions were tested: control (control), laser (L1 and L2), phenothiazine (F1 and F2), and PACT (F1L1 and F2L2). Statistically significant diferences were seen between groups (L-F +) and (F + L +) for both conditions of the laser, with a growth inhibition of the yeast around 67 and 51%, respectively, however, when using only the laser there was an increase of 18% in the survival of these cells. PACT's efficacy on fluconazole-resistant C. tropicalis depended on both the time of pre-irradiation and concentration of the PTZ.

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Rafael Araújo Gomes Júnior, Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Gustavo M. P. Santos, Fernando J. P. Sampaio D.D.S., Maria F. M. Gesteira D.D.S., Fátima A. A. Zanin, Aldo Brugnera Jr., Antônio Luiz B. Pinheiro, and Marcos A. Vannier-Santos "Association phenothiazine and laser on growth of C. tropicalis fluconazole-resistant", Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 89320G (18 February 2014); https://doi.org/10.1117/12.2038566

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