In vivo micro-Raman spectroscopy of arbitrary-shaped region of interest under simultaneous reflectance confocal imaging guidance

Zhenguo Wu; Liwei Jiang; Jianhua Zhao; Haishan Zeng

doi:10.1117/12.2657150

17 March 2023 In vivo micro-Raman spectroscopy of arbitrary-shaped region of interest under simultaneous reflectance confocal imaging guidance

Zhenguo Wu, Liwei Jiang, Jianhua Zhao, Haishan Zeng

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Proceedings Volume PC12352, Photonics in Dermatology and Plastic Surgery 2023; PC1235208 (2023) https://doi.org/10.1117/12.2657150
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Confocal Raman spectroscopy (CRS) has been used for noninvasive biochemical analysis of biological tissue in vivo. Reflectance confocal microscopy (RCM) has been integrated with CRS as visual guidance to facilitate simultaneous square-shaped region or point of interest micro-Raman measurement. However, these methods do not allow the acquisition of representative Raman spectra from and only from the area that covers the target micro-structure that has various morphologies. To solve this problem, we developed a method to acquire Raman spectra from a region of interest that has an arbitrary shape under simultaneous RCM imaging guidance using a single laser source.

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Zhenguo Wu, Liwei Jiang, Jianhua Zhao, and Haishan Zeng "In vivo micro-Raman spectroscopy of arbitrary-shaped region of interest under simultaneous reflectance confocal imaging guidance", Proc. SPIE PC12352, Photonics in Dermatology and Plastic Surgery 2023, PC1235208 (17 March 2023); https://doi.org/10.1117/12.2657150

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KEYWORDS

Confocal microscopy

In vivo imaging

Raman spectroscopy

Micro raman spectroscopy

Reflectivity

Chromium

Imaging systems

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