In-fiber photo-immobilization of a bioactive surface

Elizabeth Lee; Derrick Yong; Xia Yu; Hao Li; Chi Chiu Chan

doi:10.1117/1.JBO.19.12.120502

18 December 2014 In-fiber photo-immobilization of a bioactive surface

Elizabeth Lee, Derrick Yong, Xia Yu, Hao Li, Chi Chiu Chan

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Journal of Biomedical Optics, Vol. 19, Issue 12, 120502 (December 2014). https://doi.org/10.1117/1.JBO.19.12.120502

Abstract

We demonstrate the first in-fiber light-induced bioactive biotin-functionalization via photobleaching fluorophore-conjugated biotin. Photobleaching the fluorophores generated free radicals that bind to the albumin-passivated inner surface of pure silica photonic crystal fiber. The subsequent attachment of dye-conjugated streptavidin to the bound biotin qualified the photo-immobilization process and demonstrated a potential for the construction of in-fiber macromolecular assemblies or multiplexes. Compared with other in-fiber bioactive coating methods, the proposed light-induced technique requires only a low-power light source, without the need for additional preactivation steps or toxic chemical reagents. This method, hence, enables a simple and compact implementation for potential biomedical applications.

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Elizabeth Lee, Derrick Yong, Xia Yu, Hao Li, and Chi Chiu Chan "In-fiber photo-immobilization of a bioactive surface," Journal of Biomedical Optics 19(12), 120502 (18 December 2014). https://doi.org/10.1117/1.JBO.19.12.120502

Published: 18 December 2014

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