For measuring UV-VIS spectra of a micro-area, an upright triocular UV microscope has to be generally used and
reequipped by the user. This greatly increased the cost of the experimental system and might make the microscope lose
the function of taking a photograph. In this paper, a new micro-spectroscopy system is established for measuring
fluorescence of a micro-area. This system includes in a reflective microscope objective(RMO), a fiber cable, a microlens,
a 3D adjustable fiber-microlens-microscope adaptor(FMMA), a fluorescence meter and a common inverted fluorescence
microscope(IFM). The cheap and common IFM replaced an expensive UV upright microscope and no need to rebuild the
microscope itself. Except for the FMMA, all the other elements are standard products. All elements are easily integrated
into a whole, so the cost of the system is greatly decreased. The space resolution of this system is 2μm. This system can
be used to localize a micro-area, excite it with a monochromatic light, take a photograph of it, collect wake fluorescence
of it and measure its UV-VIS spectra. This system is stable and has a high sensitivity. It is promising to measure the
fluorescence of a mineral grain, the organic matters in a micro-fracture, or a single fluid inclusion. Because it used an
IFM, it can also to be used to the biology samples, such as cell or bio-inclusion. We used this system to measure the UVVIS
spectrum of oils in a single micro-fracture of a mineral slice and the spectra of a single mineral grain in a mineral
slice.
A focused laser light sheet was used to illuminate the capillary tube filled with liquid and fringe patterns can be seen.
The fringe patterns can be formed by rays directly passing through the capillary tube or reflected one time or two times
by the walls of the capillary tube then transmitted again. The fringe patterns formed by transmission light lie before the
capillary tube and can be bell-shaped, steep bell-shaped, or almost parallel, which strongly depend on the position of the
capillary tube. The bell shaped pattern can be qualitatively explained by the addition of diffraction of multi-slits and is
relative to the aberration of the cylindrical lens. This pattern is also relative to the refractive index of liquid containing in
the capillary tube. The fringe patterns caused by reflected light lie before or after the capillary tube, which will influence
the contrast of the bell shaped fringe pattern formed by transmitted light.
The mechanism of capillary tube interferometer is expected to be two-beam interference by ray tracing. A computer
program to simulate the interference fringe pattern was established. By comparing the simulated fringe pattern and
experimental fringe pattern, the refractive index of the liquid can be given when the two fringes coincide best. The
results of this method are very near the Abbe refratometer.
In the first time, the refractive indices of the low transparent extraction of source rocks were measured by capillary tube
interferometer. A curve between the refractive indices of the diluted extraction of source rocks and corresponding
vitrinite reflectance Ro was established. When Ro is in the range of 0.36-1.25, the refractive indices of the extraction
increase with Ro, or the maturity of the source rocks. Good correlation was observed between the refractive indices and
vitrinite reflectance Ro. The refractive index of the extraction of source rock is valuable for determining the degree of
maturity of source rock. This technique is promising to measure the refractive indices of low transparent liquids and
could be used to estimate the maturity of source rock.
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