Dr. Anna Meletiou Profile

Dr. Anna Meletiou

at Univ of Exeter

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Proceedings Article | 4 October 2020 Presentation

New DNA-based localisation microscopy approaches to study protein-protein interactions and nanoscale protein placement in situ

Alex Clowsley, William Kaufhold, Tobias Lutz, Anna Meletiou, Lorenzo Di Michele, Christian Soeller

Proceedings Volume 11575, 1157507 (2020) https://doi.org/10.1117/12.2584755

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It is crucial to visualize biomolecules and their molecular-interaction complexes directly within cells, to show precisely where these interactions occur and thus improve our understanding of cellular regulation. In this presentation I will present new approaches to image proteins and protein-protein interactions that are based on synthetic DNA interactions and allow localisation with ~10 nm precision. Two schemes will be presented, called PD-PAINT (proximity-dependent PAINT) and Repeat DNA-PAINT which are improvements of the localisation microscopy technique called DNA-PAINT. I will describe the basis of these approaches and demonstrate their application to biophysical questions in cardiac biophysics. We show that these schemes can be straightforwardly integrated in a multiplexed super-resolution imaging protocol and benefit from advantages of DNA-based super-resolution localization microscopy, such as high specificity, high resolution and the ability to image quantitatively.

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