Dr. Bob Carr Profile

Dr. Bob Carr

at NanoSight Ltd

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Proceedings Article | 3 October 2008 Paper

A reagentless real-time method for the multiparameter analysis of nanoparticles as a potential 'trigger' device

Bob Carr, John Knowles, Jeremy Warren

Proceedings Volume 7116, 71160N (2008) https://doi.org/10.1117/12.799627

KEYWORDS: Particles, Nanoparticles, Statistical analysis, Light scattering, Motion analysis, Scattering, Laser scattering, Video, Dynamic light scattering, Refractive index

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We describe the continuing development of a laser-based, light scattering detector system capable of detecting and analysing liquid-borne nanoparticles. Using a finely focussed and specially configured laser beam to illuminate a suspension of nanoparticles in a small (250ul) sample and videoing the Brownian motion of each and every particle in the detection zone should allow individual but simultaneous detection and measurement of particle size, scattered light intensity, electrophoretic mobility and, where applicable, shape asymmetry. This real-time, multi-parameter analysis capability offers the prospect of reagentlessly differentiating between different particle types within a complex sample of potentially high and variable background. Employing relatively low powered (50-100mW) laser diode modules and low resolution CCD arrays, each component could be run off battery power, allowing distributed/remote or personal deployment. Voltages needed for electrophoresis measurement s would be similarly low (e.g. 20V, low current) and 30second videos (exported at mobile/cell phone download speeds) analysed remotely. The potential of such low-cost technology as a field-deployable grid of remote, battery powered and reagentless, multi-parameter sensors for use as trigger devices is discussed.

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