Ms. Christine Tse Profile

Christine Tse

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Publications (4)

Proceedings Article | 13 February 2007 Paper

Metal/dendrimer nanocomposites for enhanced optical breakdown: acoustic characterization and initial targeted cell uptake study

Christine Tse, Wojciech Lesniak, Lajos P. Balogh, Jing Yong Ye, Matthew O'Donnell

Proceedings Volume 6449, 64490C (2007) https://doi.org/10.1117/12.698780

KEYWORDS: Silver, Nanocomposites, Metals, Acoustics, Luminescence, Dendrimers, Laser damage threshold, Backscatter, Particles, Confocal microscopy

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Metal/dendrimer nanocomposites (DNCs) uniquely combine the properties of metallic clusters and the biofriendly polymer host in a nanosized hybrid particle. DNCs can biochemically target tissues and locally reduce femtosecond optical breakdown thresholds, making highly precise and selective photodisruption possible. In this study, we have used high-frequency acoustic monitoring of bubble production dynamics to investigate how DNC properties, solution concentration, and optical parameters affect threshold reduction, actual waiting time, and mechanical characteristics of breakdown. Breakdown is defined here as bubble production with an onset of less than 20 seconds after laser exposure. DNC properties varied include metal content (silver, gold) and terminal group (amino-NH₂, glycidol-OH, and carboxyl- COOH) which determine pH values. Results indicate that DNC metal content markedly influences solution threshold reduction, while DNC terminal group (and thus net surface charge) and solution concentration influence the details of breakdown at these reduced threshold fluences. {Ag(0)} DNCs reduce breakdown threshold fluence 1-2 orders of magnitude more than {Au(0)} DNCs. Furthermore, concentrated DNC solutions and DNCs carrying a net negative charge (carboxyl terminal groups) increase bubble production up to four times and shorten waiting time for breakdown from seconds to milliseconds. Increasing laser fluence for a given DNC solution concentration also shortens breakdown waiting time. Lastly, utilizing the fluorescence properties of silver nanocomposites, we use confocal microscopy to examine KB cell uptake of folate targeted silver DNCs. Cells incubated with folate targeted silver DNCs exhibit a measurable increase of intracellular fluorescence compared to control cells (no DNC incubation). However, while we observe a threshold reduction in KB cells incubated with 500nM folate-targeted DNC solution, there is no threshold reduction in cells incubated with 50nM folate-targeted DNC solution. This suggests that a specific minimum DNC concentration may be required for localized reduced-threshold breakdown to occur.

Proceedings Article | 7 March 2006 Paper

Subsurface photodisruption in pig skin as monitored by high-frequency ultrasound

Christine Tse, Marwa Zohdy, Jing Yong Ye, Matthew O'Donnell

Proceedings Volume 6086, 60861U (2006) https://doi.org/10.1117/12.645327

KEYWORDS: Skin, Tissues, Tissue optics, Scattering, Objectives, Femtosecond phenomena, Light scattering, Ultrasonography, Laser scattering, Ultrasonics

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Proceedings Article | 25 April 2005 Paper

Optical and acoustical monitoring of femtosecond laser-induced intracellular contrast agents: initial cell culture studies

Marwa Zohdy, Christine Tse, Jing Yong Ye, Lajos Balogh, Theodore Norris, Matthew O'Donnell

Proceedings Volume 5697, (2005) https://doi.org/10.1117/12.589687

KEYWORDS: Acoustics, Nondestructive evaluation, Femtosecond phenomena, Optical microscopy, Transducers, Target detection, Laser optics, Pulsed laser operation, Camera shutters, Microscopy

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Proceedings Article | 28 April 2004 Paper

Monitoring LIOB-induced bubble characteristics in gelatin using high-frequency ultrasound

Christine Tse, Marwa Zohdy, Jing Yong Ye, Theodore Norris, Lajos Balogh, Kyle Hollman, Matthew O'Donnell

Proceedings Volume 5373, (2004) https://doi.org/10.1117/12.534092

KEYWORDS: Pulsed laser operation, Backscatter, Acoustic emission, Ultrasonography, Laser stabilization, Laser optics, Laser tissue interaction, Tissue optics, Laser damage threshold, Cavitation

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