To investigate the optimal gold colloid film properties for the in-situ detection of polycyclic aromatic hydrocarbon
(PAH) in water using 671 nm excitation wavelength, gold substrates with selected sizes of gold nanoparticles were
prepared. Heating of (3-aminopropyl)trimethoxysilane (APTMS) methanol solution allowed to control the surface
plasmon resonance for surface enhanced Raman spectroscopy (SERS). Shifted excitation Raman difference spectroscopy
(SERDS) was applied for effective background removal. Results for pyrene in aqueous solution demonstrate that there is
a dependence of SERS activity on the size of gold nanoparticles even though their surface plasmon resonances are
almost same and close to the excitation wavelengths, and also confirmed that the improved self-assembly method using
controlling the temperature of APTMS solution way can work well. Appling the substrate with the average gold
nanoparticles size of 35 nm, different concentrations of pyrene in aqueous solution were detected with SERS supported
by SERDS technique. Raman signals of pyrene up to 5 nmol/l on this substrate can be obtained. The quantitative analysis
shows the SERS intensities increase with the increasing concentration of pyrene, which is the foundation of quantitative
analysis of pyrene in aqueous solution.
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