Ultraviolet light-induced damage to human skin DNA was widely investigated. The primary mechanism of this damage
contributed to form cyclobutane pyrimidine dimmers (CPDs). Although the distribution of UV light-induced CPDs
within a defined sequence is similar, the damage in cellular environment which shields the nuclear DNA was higher than
that in organism in apparent dose. So we use low UVB light as main study agent. Low dose UV-irradiated HDF-a cells
(Human Dermal Fibroblasts-adult cells) which is weaker than epidermic cells were cultured with DMEM at different
trace amount of Mg2+ (0mmol/L , 0.1mmol/L , 0.2mmol/L, 0.4mmol/L, 0.8mmol/L, 1.2mmol/L) free-serum DMEM and
the repair of DNA strands injured were observed. Treat these cells with DNA strand breaks detection, photoproducts
detection and the repair of photoproducts detection. Then quantitate the role of trace amount Mg2+ in repair of UV
light-induced damage to human skin. The experiment results indicated that epidermic cells have capability of resistance
to UV-radiation at a certain extent. And Mg2+ can regulate the UV-induced damage repair and relative vitality. It can
offer a rationale and experiment data to relieve UV light-induced skin disease.
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