In this study, the GB-speckles were used to study the probable precursor for L. monocytogenes ST734 strains, which were detected twice in the world, namely: for the first time in Chile in 2009 and, recently, in 2019 in the Republic of Serbia. We found that these strains belong to the clonal complex I and differ fromL. monocytogenes ST1 strains by a single nucleotide change in the gene dat. This is the first report demonstrating the using GB-speckle-structures to detect evolutionary genetic changes in L. monocytogenes strains.
Our previous study has revealed that specific ornithosis antigens extracted from Сhlamydia psittaci (CP) could possess some modulatory activity on the growth of malignant solid tumors in Wistar rats. Here, in the same model we studied dose-dependent dynamics of tumor growth under the influence of CP antigen derived from CP AMK-16 strain, in doses ranged from 0.025 mg/kg to 1.25 mg/kg given as a single subcutaneous injection. Velocity of tumor growth has been estimated by t-LASCA technique.
The nucleotide sequences of the neuraminidase gene of Highly Pathogenic Avian Influenza Virus (HPAIV) A strains have been transformed into 2D realizations of GB-speckles (Gene-Based speckles). Possibility of differentiation between different HPAIV strains using the analysis of statistical properties of corresponding GB-speckles has been shown.
Chlamydiaceae family is consisted of intracellular bacteria pathogenic for both humans and animals. In this paper the nucleotide sequences of the gene encoding Pgp4 protein, the well-known virulence factor in Chlamydia, have been successfully transformed into the form of the GB-speckles. Strong discrimination was found between the relevant speckle patterns derived from three Chlamydiae spp., namely Chlamydia psittaci, Chlamydia abortus and Chlamydia trachomatis.
The gene "N" encodes the nucleocapsid structural protein of SARS–CoV-2 virus COVID-19. In the present time, nucleocapsid protein is one of the important targets for the study of both humoral and cellular immune responses to SARS–CoV-2. In this study sequence of the SARS-CoV-2 gene "N", which is encoding the relevant structural protein, has been transformed into the sequence of numbers with purpose to generate gene-based speckles. Differences in the initial nucleotide sequences have been found and characterized by means of virtual laser gene-based speckles (GBspeckles). It has been shown, that at the interference of two SARS–CoV-2 GB-speckle-patterns, two types of interference pictures are forming: quasi-random speckle structure without interference fringes or fringes, modulated by speckles. It has been demonstrated that implication of interference of two GB-speckles, generated on the virus nucleotide sequences, can be considered as a new direction in modern bioinformatics.
Chlamydia trachomatis biodiversity has been detected in clinical urogenital samples of Chlamydia patients in the Southeastern European Region of Russia. In this paper three absolutely new nucleotide sequences of “Saratov” strains (Saratov E1/61.8-B1, Saratov E2/61.46-L38, Saratov E6/61.35-B1) have been described and transformed into the form of GB-speckles. It has been demonstrated that identification of single polymorphism in nucleotide sequences can be easily performed on the base of GB-speckles. Procedure of detection of polymorphism can be made essentially faster in the case of using of Matlab Parallel Computing Toolbox.
New method of photoinactivation of plague microbes (bacteria Yersiniapestis) has been suggested. Rate of growth of colonies of Y.pestis EV NIIEG at specific regimes of photo processing have been analyzed. Dependence of growth on exposure time and concentrations of photosensitizer (methylene blue) has been studied. Number of colony forming units of Y.pestis EV NIIEG bacteria as a function of intensity of light and concentration of methylene blue has been scrutinized.
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