Mr. Mohammed A. Alfadhel Profile

Mohammed A. Alfadhel

at Athinoula A Martinos Ctr for Biomedical Imaging

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Publications (9)

SPIE Journal Paper | 25 January 2025 Open Access

Low-cost physiology and behavioral monitor for intravital imaging in small mammals

Yuntao Li, Alfredo Cardenas-Rivera, Chang Liu, Zhengyi Lu, Jaime Anton, Mohammed Alfadhel, Mohammad Yaseen

NPh, Vol. 12, Issue 01, 015004, (January 2025) https://doi.org/10.1117/12.10.1117/1.NPh.12.1.015004

KEYWORDS: Animals, Cameras, Sensors, Brain, Blood, Imaging systems, Calcium, Physiology, Fluorescence, Data acquisition

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Proceedings Article | 13 March 2024 Presentation

Capillary blood flow imaging in awake mice using dynamic light scattering OCT and two photon microscopy

Sida Liang, Qi Pian, Mohammed Alfadhel, Buyin Fu, Jianbo Tang, Sava Sakadzic

Proceedings Volume PC12841, PC128410F (2024) https://doi.org/10.1117/12.3023450

KEYWORDS: Blood circulation, Capillaries, Two photon excitation microscopy, Optical coherence tomography, Dynamic light scattering, Biological imaging, Animal model studies, Red blood cells, Animals, Velocimetry

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SPIE Journal Paper | 21 July 2023 Open Access

Cortical microvascular blood flow velocity mapping by combining dynamic light scattering optical coherence tomography and two-photon microscopy

Qi Pian, Mohammed Alfadhel, Jianbo Tang, Grace V. Lee, Baoqiang Li, Buyin Fu, Yagmur Ayata, Mohammad Abbas Yaseen, David A. Boas, Timothy W. Secomb, Sava Sakadzic

JBO, Vol. 28, Issue 07, 076003, (July 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.7.076003

KEYWORDS: Angiography, Optical coherence tomography, Capillaries, Blood circulation, Image segmentation, Brain, Biological imaging, Data acquisition, Voxels, Velocity measurements

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SignificanceThe accurate large-scale mapping of cerebral microvascular blood flow velocity is crucial for a better understanding of cerebral blood flow (CBF) regulation. Although optical imaging techniques enable both high-resolution microvascular angiography and fast absolute CBF velocity measurements in the mouse cortex, they usually require different imaging techniques with independent system configurations to maximize their performances. Consequently, it is still a challenge to accurately combine functional and morphological measurements to co-register CBF speed distribution from hundreds of microvessels with high-resolution microvascular angiograms.AimWe propose a data acquisition and processing framework to co-register a large set of microvascular blood flow velocity measurements from dynamic light scattering optical coherence tomography (DLS-OCT) with the corresponding microvascular angiogram obtained using two-photon microscopy (2PM).ApproachWe used DLS-OCT to first rapidly acquire a large set of microvascular velocities through a sealed cranial window in mice and then to acquire high-resolution microvascular angiograms using 2PM. The acquired data were processed in three steps: (i) 2PM angiogram coregistration with the DLS-OCT angiogram, (ii) 2PM angiogram segmentation and graphing, and (iii) mapping of the CBF velocities to the graph representation of the 2PM angiogram.ResultsWe implemented the developed framework on the three datasets acquired from the mice cortices to facilitate the coregistration of the large sets of DLS-OCT flow velocity measurements with 2PM angiograms. We retrieved the distributions of red blood cell velocities in arterioles, venules, and capillaries as a function of the branching order from precapillary arterioles and postcapillary venules from more than 1000 microvascular segments.ConclusionsThe proposed framework may serve as a useful tool for quantitative analysis of large microvascular datasets obtained by OCT and 2PM in studies involving normal brain functioning, progression of various diseases, and numerical modeling of the oxygen advection and diffusion in the realistic microvascular networks.

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SPIE Journal Paper | 14 June 2023 Open Access

Differential reductions in the capillary red-blood-cell flux between retina and brain under chronic global hypoperfusion

Baoqiang Li, Ji Leng, Ikbal Şencan-Eğilmez, Hajime Takase, Mohammed Ali H. Alfadhel, Buyin Fu, Mahnaz Shahidi, Eng Lo, Ken Arai, Sava Sakadžić

NPh, Vol. 10, Issue 03, 035001, (June 2023) https://doi.org/10.1117/12.10.1117/1.NPh.10.3.035001

KEYWORDS: Capillaries, Retina, Red blood cells, Brain, Animals, Animal model studies, Brain diseases, Blood circulation, Biological imaging, Retinal diseases

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Proceedings Article | 25 April 2023 Poster + Paper

LPS-induced inflammation alters cerebral oxygenation but not blood flow in a mouse model of Alzheimer’s disease

Chang Liu, Mohammed Alfadhel, Shayna Teitelbaum, Austin Birmingham, Alfredo Cardenas-Rivera, Mohammed Yaseen

Proceedings Volume 12384, 123840L (2023) https://doi.org/10.1117/12.2647187

KEYWORDS: Capillaries, Brain, Oxygen, Inflammation, Red blood cells, Oxygenation, Phosphorescence, Blood circulation, Alzheimer disease, Photons

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Proceedings Article | 28 April 2022 Presentation

Effects of voluntary exercise on cerebral microvascular oxygenation in aged mice

Paul Shin, Qi Pian, Hidehiro Ishikawa, Gen Hamanaka, Emiri Mandeville, Guo Shuzhen, Buyin Fu, Mohammed Alfadhel, Ikbal Sencan, Baoqiang Li, Chongzhao Ran, Sergei Vinogradov, Eng Lo, Ken Arai, Anna Devor, Sava Sakadžic

Proceedings Volume PC11946, PC119460S (2022) https://doi.org/10.1117/12.2610303

KEYWORDS: Capillaries, Two photon excitation microscopy, Oxygen, Brain

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Proceedings Article | 5 March 2021 Poster

Two-photon microscopic measurements of the effect of bilateral carotid artery stenosis on the cerebral and retinal microcirculation

Baoqiang Li, Ikbal Sencan, Hajime Takase, Ji Leng, Mohammed Alfadhel, Martin Thunemann, Stephen Adams, Jing Yang, Buyin Fu, Chongzhao Ran, David Boas, Anna Devor, Mahnaz Shahidi, Eng Lo, Ken Arai, Sava Sakadžic

Proceedings Volume 11629, 116292N (2021) https://doi.org/10.1117/12.2579392

KEYWORDS: Arteries, Retina, Capillaries, Brain, Neuroimaging, Nervous system, Mouse models, Luminescence, Ischemia, Blood circulation

Proceedings Article | 5 March 2021 Presentation

Large scale mapping of the cortical microvascular velocities by the dynamic light scattering optical coherence tomography

Mohammed Alfadhel, Qi Pian, Jianbo Tang, Grace Lee, Baoqiang Li, Buyin Fu, Yagmur Ayata, David Boas, Timothy Secomb, Sava Sakadžic

Proceedings Volume 11629, 116290Z (2021) https://doi.org/10.1117/12.2583146

KEYWORDS: Optical coherence tomography, Dynamic light scattering, Blood circulation, Oxygen, Angiography, Two photon excitation microscopy, Quantitative analysis, Numerical modeling, Image segmentation, Diffusion

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Proceedings Article | 5 March 2021 Presentation

Two-photon phosphorescence lifetime microscopy of intestinal capillary pO2 and RBC flux

Ikbal Sencan, Wenjing Zong, Mohammed Alfadhel, Buyin Fu, Mohammad Yaseen, Gary Wu, Sava Sakadzic, Sergei Vinogradov

Proceedings Volume 11648, 116480H (2021) https://doi.org/10.1117/12.2583123

KEYWORDS: Capillaries, Phosphorescence, Microscopy, Oxygen, Intestine, Hyperoxia, Body temperature, Tissues, Natural surfaces, Glasses

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Showing 5 of 9 publications

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