Fluorescence labelling is one of the significant properties for optical bioimaging and biosensing applications. The development in the field of nanotechnology has created enormous conveniences in biomedical research. The fluorescence enhancement of mammalian cell in presence of zinc nitride colloidal nanoparticle was imaged using multiphoton confocal microscope. The statistical significance in the presence and absence of nanoparticle evidenced the fluorescence intensity enhancement in the cells. In order to suppress the background signal and increase the penetration depth, the multiphoton technique is utilized by 740 nm excitation to examine the enhancement potential of Zn3N2 colloidal nanoparticles towards cell structures. Hence, we suggest that, the unique feature of inorganic nanomaterial is a promising material for probing cells for future diagnostic applications.
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