The 3D fabrication of bio-compatible materials under an aqueous environment is a fundamental requirement for tissue
engineering. In this work, a series of polyethylene glycol- (PEG-), carboxylate anionic group- or pyridyl cationic groupfunctionalized
benzylidene cyclopentanone photo-initiators (B2, B3, X2, X3, Y1 and P1) were synthesized. Their
water-solubility, photophysical properties and cytotoxicity were investigated. Using eosin as reference, their initiation
efficiencies in water-soluble photoactive formulation (SR610 with 20% of DI water) excited by one- or two-photon were
studied. The results showed that there were no significant differences on partial linear photophysical properties of these
initiators, such as [see manuscript] and [see manuscript], but differences on their (see manuscript), Φ, and σmax are clear. In addition, their cytotoxicity
is different. Two PEG-functionalized initiators (B3 and X3) with two PEG groups on one side of benzylidene
cyclopentanone cores have obvious toxicity, while other four intiators are safety to HepG2 cells at the concentration of
20 μM. Moreover, the water-solubility of X2 and X3 (containing two longer PEG) were much better than those of other
four initiators. Using these initiators, 3D micro-structures fabricated by two-photon polymerization of water-soluble
acrylate all could be achieved. In addition, their threshold energies were all lower than 0.5 mW. However, the stiffness of
3D micro-structures was affected by the water-solubility of these photo-initiators. Those structures built by formulations
containing X2 and X3 would collapse easily. Conversely, other structures could sustain very well. This work proves that
B2,Y1,and P1 have extensive application prospects in 3D fabrication for tissue engineering.
A series of amphiphilic benzylidene cycloalkanes ketone photosensitizers C1-C4 with or without folate receptor-targeted
agent were designed and synthesized. Their photophysical properties and in vitro photodynamic therapy (PDT) effects
were studied. The results showed that all compounds exhibited appropriate lipid-water partition coefficients and high
reactive oxygen yields. The introduction of the folate receptor-targeted agent had no obvious influence on the basic
photophysical & photochemical properties of C2 and C4 compared to those of their corresponding prototype compounds
(C1 and C3). In vitro studies were carried out using MCF-7 cells (FR+), Hela cells (FR+) and A549 cells (FR-), which
represented different levels of folate receptor (FR) expression. All of C1-C4 showed low dark toxicity and superior PDT
effects compared with the clinical drug PSD-007 (a mixture of porphyrins). What’s more, folate receptor-targeted
photosensitizers (C2 and C4) achieved higher accumulation and more excellent PDT effects in MCF-7 cells (FR+) and
Hela cells (FR+) than photosensitizers (C1 and C3) without folate receptor-targeted agent and PSD-007. The photocytotoxicity
of these photosensitizers showed no obvious differences in A549 cells (FR-).
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