Ms. Ya Wang Profile

Ya Wang

at Columbia Univ

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Proceedings Article | 29 April 2005 Paper

Recognition of micro-array protein crystals images using multi-scale representations

Ya Wang, David Kim, Elsa Angelini, Andrew Laine

Proceedings Volume 5747, (2005) https://doi.org/10.1117/12.595902

KEYWORDS: Crystals, Proteins, Image classification, Image processing, Radon transform, Image filtering, Hough transforms, Neural networks, Databases, Feature extraction

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Micro-array protein crystal images are now routinely acquired automatically by CCD cameras. High-throughput automatic classification of protein crystals requires to alleviation of the time-consuming task of manual visual inspection. We propose a classification framework combined with a multi-scale image processing method for recognizing protein crystals and precipitates versus clear drops. The main two points of the processing method are the multi-scale Laplacian pyramid filters and histogram analysis techniques to find an effective feature vector. The processing steps include: 1. Tray well cropping using Radon Transform; 2. Droplet cropping using an ellipsoid Hough Transform; 3. Multi-scale image separation with Laplacian pyramidal filters; 4. Feature vector extraction from the histogram of the multi-scale boundary images. The feature vector combines geometric and texture features of each image and provides input to a feed forward binomial neural network classifier. Using human (expert crystallographers) classified images as ground truth, the current experimental results gave 86% true positive and 94% true negative rates (average true percentage is 90%) using an image database which contained over 2,000 images. To enable NESG collaborators to carry our crystal classification, a web-based Matlab server was also developed. Users at other locations on the internet can input micro-array crystal image folders and parameters for training and testing processes through a friendly web interface. Recognition results are shown on the client side website and may be downloaded by a remote user as an Excel spreadsheet file.

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